Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri
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Paramagnetic centers of carbon monoxide dehydrogenase from aceticlastic Methanosarcina barkeri.
Carbon monoxide dehydrogenase from Methanosarcina barkeri, purified to 95% homogeneity, contains 30 Fe, 2 Ni, 1 Zn, and 1 Cu (per alpha 2 beta 2 enzyme). Core extrusion experiments indicate 6 [4Fe-4S] clusters/tetramer, and electron paramagnetic resonance (epr) spectroscopy detects at least one of these clusters, in the reduced form, with apparent g values of 2.05, 1.94, and 1.90, and Em9.2-390...
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Conversion of acetate to methane (aceticlastic methanogenesis) is an ecologically important process carried out exclusively by methanogenic archaea. An important enzyme for this process as well as for methanogenic growth on carbon monoxide is the five-subunit archaeal CO dehydrogenase/acetyl coenzyme A (CoA) synthase multienzyme complex (CODH/ACS) catalyzing both CO oxidation/CO(2) reduction an...
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Title of Document: ARCHAEAL TRANSCRIPTIONAL REGULATION OF CATABOLIC CARBON MONOXIDE DEHYDROGENASE IN METHANOSARCINA SPECIES Kimberly Lynn Anderson, Doctor of Philosophy, 2009 Directed By: Professor Kevin R. Sowers, Marine Estuarine Environmental Sciences In Archaea, the basal transcription machinery is eukaryotic-like, but some components, such as activator and repressor proteins, are bacteria-...
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The carbon monoxide dehydrogenase from the photosynthetic bacterium Rhodospirillum rubrum was purified over 600-fold by DEAE-cellulose chromatography, heat treatment, hydroxylapatite chromatography, and preparative scale gel electrophoresis. In vitro, this enzyme catalyzed a two-electron oxidation of CO to form CO2 as the product. The reaction was dependent on the addition of an electron accept...
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The conversion of carbon sources such as coal and methane into liquid fuels, methanol and alkanes, the latter via the Fisher-Tropsch process, requires careful control of the H2/CO ratio in synthesis gas. 1 The control is achieved in industry by the water-gas shift reaction. (Figure 1). 2 The study of carbon monoxide dehydrogenase (CODH) enzymes is significant to the water gas shift reaction bec...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1984
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.158.1.231-237.1984